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The cooler temperatures in the other water bath should cause the rate to be much slower in all three respirometers. Materials A Water bath, thermometer, masking tape, washers, beads, germinating peas, non-germinating peas, beakers, graduated cylinder, ice, paper, and pencil are needed for this lab.

Methods Begin the experiment by setting up two water baths, one at room temperature and the other at 10 degrees Celsius. Next, find the volume of germinating peas, non- germinating peas and bead, and beads alone. Repeat these steps for another set of peas and beads. Assemble the six respirometers, placing enough KOH pellets to cover the bottoms of the respirometers.

Put non-absorbent cotton balls in each respirometer above the KOH pellets and then add the peas and beads. Place one set of respirometers in the room temperature water bath and the other set into the 10 degree water bath. Slightly elevate the respirometers, supporting them with masking tape, for 5 minutes while they equilibrate. Then lower the respirometers into the water bath and take a reading at 5, 10, 15, 20, 25, and 30 minute time intervals.

Record the data into the table. Data: Questions 1. In this activity, you are investigating both the effect of germination versus non-germination and warm temperature versus cold temperature on respiration rate. Germinating peas should consume more oxygen than non-germinating peas. Peas germinating at warm temperatures should consume more oxygen than peas germinating at cold temperatures. This activity uses a number of controls.

Identify at least three of the control, and describe the purpose of each control. Water baths held at constant temperature Volume of KOH is the equal in every tube Equilibration time is identical for all respirometers.

Graph the results from the corrected difference column for the germinating peas and dry peas at both room temperature and 10 degrees Celsius. Describe and explain the relationship between the amount of oxygen consumed and time. The amount of oxygen consumed was greatest in germinating peas in warm water.

The oxygen consumption increased over time in germinating peas. Complete the following table:. Do the number of months off Southern Chlor sodium hydroxide divided 12 which gives us 0. No, he wasn't ideal guests. A question we need to calculate. What is the moderate off air going into the problem? It's true that the Mueller rate off. Yeah, going into the problem keys Physical depression volume developed by RT.

The consecration is giving him. Therefore we need to get the absolute pressure we need to end 10, 10, millimeter off water with with limited off water. Let's do that and let's converted to advance. Very pressure.

Therefore ready to divide this number by again 10, and the volume given her is to leader. Finally, yes. Constant is 0. Mullingar Song, Calvin and we're Strong Temperature. Even here is doing to treat in the sense, yes, this kind of Kelvin. Therefore, we get the moderate off. They're going into the bubbler is there when the 14 minutes. Now we can calculate What is the Mueller concentration off?

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In chemistry and physics, an element is a substance that cannot be broken down into a simpler substance by chemical means. A pure element is a substance consisting of a single type of atom, with its chemical properties determined by that atom's atomic number, which is the number of protons in its nucleus.

Examples of elements include carbon, oxygen, aluminum, iron, gold, copper, mercury, and lead. In biology, the elements of life are the essential building blocks that make up living things. They are carbon, hydrogen, nitrogen, oxygen, phosphorus, and sulfur. The first four of these are the most important, as they are used to construct the molecules that are necessary to make up living cells.

These elements form the basic building blocks of the major macromolecules of life, including carbohydrates, lipids, nucleic acids and proteins. Measure the water readings in intervals of five minutes for twenty minutes. The peas that have been germinating for three days will have the highest rate of respiration and the beads will have the lowest rate of respiration.

According to your graph, what happens to the rate of oxygen consumed by germinating peas over time? What does this indicate to you? The rate of oxygen consumption is fairly constant.

How did the KOH affect the water movement in the respirometer? It allows more water into the pipette. Which of the two pea types, germinating or non-germinating, consumes the most oxygen? Germinating peas consume more oxygen because they are growing and are more active than non-germinating peas. What was the effect of temperature on pea respiration?

Warmer temperatures allow for the peas to respire at a faster rate. During aerobic respiration, glucose is broken down to form several end products. Which end products contain the carbon atoms from glucose? The hydrogen atoms from glucose? The oxygen atoms from glucose? The energy stored in the glucose molecules? Carbon dioxide contains the carbon, water contains the hydrogen, both carbon dioxide and water contain the oxygen, and ATP contains the energy.

What is fermentation? What are the two types of fermentation? What organisms use fermentation? Fermentation is a catabolic process that makes a limited amount of ATP from glucose without an electron transport chain and that produces an end-product such as ethyl alcohol or lactic acid. The two types of fermentation are alcoholic and lactic acid fermentation. Plants use alcoholic while animals use lactic acid.

Draw a Venn diagram showing how respiration and fermentation are similar and how they differ. What are the three pathways involved in the complete breakdown of glucose to carbon dioxide and water? What reaction is needed to join two of these pathways? What are the substrates and products of this reaction and where does it take place? The three pathways are glycolysis, the electron transport chain, and the Krebs Cycle. Calculate the energy yield of glycolysis and cellular respiration per glucose molecule.

Distinguish between substrate-level phosphorylation and oxidative phosphorylation. Where does the energy for oxidative phosphorylation come from? Four of the ATPs are formed from substrate level and 32 from oxidative. Some of the errors that could have occurred during the experiment were water leaking into the respirometers, gas escaping the respirometers, water temperature in the bath changing, and mathematical mistakes.

This lab showed that germinating peas consumed more oxygen at a faster rate than the non-germinating peas and the beads did. It also showed that the respiration rate of germinating peas was faster than the respiration rate of non-germinating peas. Finally, this experiment showed that respiration rates increase as the temperature increases.

This shows that temperature and respiration rates are directly proportional to each other.